AffiELISA® Multiplex ELISA Kit For Mouse Cytokine Panel 1 (6-Plex)

Product Overview The Multiplex Mouse Cytokine Panel 1 (6-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of six biomarkers or analytes (IFN-γ, IL-1α, IL-1β, IL-6, IL-10, TNFα) . Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing six distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results. Using just 25 µl of sample per well, up to 80 samples can be assayed for all six markers in the panel in 5.5 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample. Assay Ranges Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IFNγ Sandwich 516 - 0.71 (pg/mL) 361 (pg/mL) 0.94 (pg/mL) 0.35 (pg/mL) IL-1α Sandwich 610 - 0.84 (pg/mL) 427 (pg/mL) 1.1 (pg/mL) 0.42 (pg/mL) IL-1β Sandwich 2500 - 3.43 (pg/mL) 1750 (pg/mL) 4.5 (pg/mL) 1.71 (pg/mL) IL-6 Sandwich 504 - 0.69 (pg/mL) 353 (pg/mL) 0.91 (pg/mL) 0.35 (pg/mL) IL-10 Sandwich 667 - 0.91 (pg/mL) 467 (pg/mL) 1.33 (pg/mL) 0.46 (pg/mL) TNFα Sandwich 429 - 0.59 (pg/mL) 300 (pg/mL) 0.69 (pg/mL) 0.29 (pg/mL) Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IFNγ Sandwich 516 - 0.71 (pg/mL) 361 (pg/mL) 0.94 (pg/mL) 0.35 (pg/mL) IL-1α Sandwich 610 - 0.84 (pg/mL) 427 (pg/mL) 1.1 (pg/mL) 0.42 (pg/mL) IL-1β Sandwich 2500 - 3.43 (pg/mL) 1750 (pg/mL) 4.5 (pg/mL) 1.71 (pg/mL) IL-6 Sandwich 504 - 0.69 (pg/mL) 353 (pg/mL) 0.91 (pg/mL) 0.35 (pg/mL) IL-10 Sandwich 667 - 0.91 (pg/mL) 467 (pg/mL) 1.33 (pg/mL) 0.46 (pg/mL) TNFα Sandwich 429 - 0.59 (pg/mL) 300 (pg/mL) 0.69 (pg/mL) 0.29 (pg/mL) IFNγ Sandwich 516 - 0.71 (pg/mL) 361 (pg/mL) 0.94 (pg/mL) 0.35 (pg/mL) IFNγ Sandwich 516 - 0.71 (pg/mL) 361 (pg/mL) 0.94 (pg/mL) 0.35 (pg/mL) IL-1α Sandwich 610 - 0.84 (pg/mL) 427 (pg/mL) 1.1 (pg/mL) 0.42 (pg/mL) IL-1α Sandwich 610 - 0.84 (pg/mL) 427 (pg/mL) 1.1 (pg/mL) 0.42 (pg/mL) IL-1β Sandwich 2500 - 3.43 (pg/mL) 1750 (pg/mL) 4.5 (pg/mL) 1.71 (pg/mL) IL-1β Sandwich 2500 - 3.43 (pg/mL) 1750 (pg/mL) 4.5 (pg/mL) 1.71 (pg/mL) IL-6 Sandwich 504 - 0.69 (pg/mL) 353 (pg/mL) 0.91 (pg/mL) 0.35 (pg/mL) IL-6 Sandwich 504 - 0.69 (pg/mL) 353 (pg/mL) 0.91 (pg/mL) 0.35 (pg/mL) IL-10 Sandwich 667 - 0.91 (pg/mL) 467 (pg/mL) 1.33 (pg/mL) 0.46 (pg/mL) IL-10 Sandwich 667 - 0.91 (pg/mL) 467 (pg/mL) 1.33 (pg/mL) 0.46 (pg/mL) TNFα Sandwich 429 - 0.59 (pg/mL) 300 (pg/mL) 0.69 (pg/mL) 0.29 (pg/mL) TNFα Sandwich 429 - 0.59 (pg/mL) 300 (pg/mL) 0.69 (pg/mL) 0.29 (pg/mL) Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Substrate A: Liquid, 3.5 mL/vial Substrate B: Liquid, 3.5 mL/vial Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips