AffiELISA® Multiplex ELISA Kit For Human Cytokine Panel 1 (6-Plex)

Product Overview The Multiplex Human Cytokine Panel 1 (6-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of six biomarkers or analytes (IL-1β, IL-6, TNFα, IFN-γ, IL-1α, IL-10) . Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing six distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results. Using only 25 µl of sample per well, up to 80 samples can be assayed for all six markers in the panel in 2.25 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample. Assay Ranges Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IFNγ Sandwich 8000 -10.97 (pg/mL) 5600 (pg/mL) 14.3 (pg/mL) 4.95 (pg/mL) IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-1β Sandwich 12226 -16.77 (pg/mL) 8558 (pg/mL) 21.8 (pg/mL) 12.40 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) IL-10 Sandwich 8039 – 11.03 (pg/mL) 5627 (pg/mL) 14.3 (pg/mL) 10.99 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IFNγ Sandwich 8000 -10.97 (pg/mL) 5600 (pg/mL) 14.3 (pg/mL) 4.95 (pg/mL) IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-1β Sandwich 12226 -16.77 (pg/mL) 8558 (pg/mL) 21.8 (pg/mL) 12.40 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) IL-10 Sandwich 8039 – 11.03 (pg/mL) 5627 (pg/mL) 14.3 (pg/mL) 10.99 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) IFNγ Sandwich 8000 -10.97 (pg/mL) 5600 (pg/mL) 14.3 (pg/mL) 4.95 (pg/mL) IFNγ Sandwich 8000 -10.97 (pg/mL) 5600 (pg/mL) 14.3 (pg/mL) 4.95 (pg/mL) IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-1β Sandwich 12226 -16.77 (pg/mL) 8558 (pg/mL) 21.8 (pg/mL) 12.40 (pg/mL) IL-1β Sandwich 12226 -16.77 (pg/mL) 8558 (pg/mL) 21.8 (pg/mL) 12.40 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) IL-10 Sandwich 8039 – 11.03 (pg/mL) 5627 (pg/mL) 14.3 (pg/mL) 10.99 (pg/mL) IL-10 Sandwich 8039 – 11.03 (pg/mL) 5627 (pg/mL) 14.3 (pg/mL) 10.99 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips