AffiELISA® Multiplex ELISA Kit For Human Female Hormone (6-plex)

Product Overview The Multiplex Human Female Hormone (6-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of six biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all six markers in the panel within 2.75 hours. The Multiplex Human Female Hormone (6-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing six distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results. Assay Ranges Analytes Calibrator Range Units LLD Adiponectin 28.3 - 0.12 ng/mL 1.64 Cortisol 744 - 1.02 ng/mL 6.45 C-Peptide 39 - 0.16 ng/mL 0.16 E1G 522 - 0.72 ng/mL 0.5 FSH 167 - 0.69 mlU/mL 2.3 hCGß 50 - 0.07 ng/mL 0.05 Analytes Calibrator Range Units LLD Analytes Calibrator Range Units LLD Analytes Calibrator Range Units LLD Adiponectin 28.3 - 0.12 ng/mL 1.64 Cortisol 744 - 1.02 ng/mL 6.45 C-Peptide 39 - 0.16 ng/mL 0.16 E1G 522 - 0.72 ng/mL 0.5 FSH 167 - 0.69 mlU/mL 2.3 hCGß 50 - 0.07 ng/mL 0.05 Adiponectin 28.3 - 0.12 ng/mL 1.64 Adiponectin 28.3 - 0.12 ng/mL 1.64 Cortisol 744 - 1.02 ng/mL 6.45 Cortisol 744 - 1.02 ng/mL 6.45 C-Peptide 39 - 0.16 ng/mL 0.16 C-Peptide 39 - 0.16 ng/mL 0.16 E1G 522 - 0.72 ng/mL 0.5 E1G 522 - 0.72 ng/mL 0.5 FSH 167 - 0.69 mlU/mL 2.3 FSH 167 - 0.69 mlU/mL 2.3 hCGß 50 - 0.07 ng/mL 0.05 hCGß 50 - 0.07 ng/mL 0.05 Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Competitor: Liquid, HRP conjugated competitive antigen Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips