AffiASSAY® Glutamate Assay Kit (Fluorometric)

Glutamate (Glutamic Acid, Glu) is one of the most abundant proteogenic amino acids in humans. It is a key molecule in cellular metabolism. For example, Glutamate plays an important role both in the metabolism of some amino acids and disposal of excess nitrogen in the form of urea. In addition, Glutamate is the main excitatory neurotransmitter in mammalian nervous system and involved in learning, cognitive and memory processes. Glutamate signaling activates receptors which have been implicated in mental disorders (Schizophrenia), neurodevelopment disorders (autism) and Neurodegenerative diseases like Alzheimer’s and Parkinson’s disease. BioVision’s PicoProbeTM Glutamate Assay Kit (Fluorometric) allows for quantification of Glutamate in Biological Fluids and Tissues. The assay is based on an enzymatic reaction in which a fluorogenic probe is reduced producing a stable signal. The reduced fluorophore produces a strong signal (Ex/Em= 535/587 nm), which is directly proportional to the amount of Glutamate in samples. The assay is simple, reproducible, and can specifically detect as low as 5 pmol of Glutamate in a 100 µl reaction