The PKA alpha/beta CAT (Phospho-Thr197) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PKA alpha/beta CAT (Phospho-Thr197) protein expression profile in cells. The kit can be used for measuring the relative amounts of PKA alpha/beta CAT (Phospho-Thr197) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PKA alpha/beta CAT (Phospho-Thr197) .