AffiASSAY® Phosphoenolpyruvate Carboxykinase Activity Assay Kit (Colorimetric)

Phosphoenolpyruvate Carboxykinase (PEPCK EC 4.1.1.32) is an enzyme which belongs to the lyase family. In the presence of GTP, it catalyzes the reversible conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), GDP and CO2. In humans, two isoforms of PEPCK are found: cytosolic form (PEPCK-C, also called PCK1) and mitochondrial form (PEPCK-M). PEPCK-C is a rate-controlling step in gluconeogenesis. Recent studies found abnormal concentrations of PEPCK in diabetic mice. Therefore, accurate measurement of Phosphoenolpyruvate Carboxykinase activity is valuable for both, mechanistic and therapeutic studies. BioVision’s Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay kit provides a quick and easy way for measuring PEPCK activity in various samples. In this assay, Phosphoenolpyruvate Carboxylase is coupled with a set of enzymes that convert PEP and carbonate into a series of intermediates and hydrogen peroxide, which in turn, reacts with a probe and converted generating a colorimetric signal (OD: 570 nm). The color intensity is directly proportional to the amount of active Phosphoenolpyruvate Carboxylase present in samples. The assay is simple, sensitive, high-throughput adaptable and can detect less than 10 µU of Phosphoenolpyruvate Carboxykinase activity per sample