AffiASSAY® Phenylalanine Colorimetric Assay Kit

L-Phenylalanine (Phe) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids building the polypeptide backbone. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in Phe metabolism lead to phenylketonuria or PKU which can have dire consequences. BioVision’s Phenylalanine Assay Kit (Colorimetric) provides a quick, simple, accurate method for quantifying Phe in biological samples. In the assay, Phe is metabolized with the simultaneous formation of NADH which reacts with a probe to generate absorbance that can be followed colorimetrically at 450 nm. The assay is linear in the range from 0.2 to 10 nmol, allowing accurate determination of concentrations in the range of 1 - 50 µM Phenylalanine.